KEITH D. GARLID, M.D., PhD

February 3, 2003

Education

Norwegian Institute of Technology, Trondheim, Norway, 1987, Dr. technicae norwegiensis (Physical Chemistry)

The Johns Hopkins University School of Medicine, 1956-1961, M.D.

Gustavus Adolphus College, 1952-1956, B.A. (Chemistry)

Employment

2001-present	Professor, Department of Biology, Portland State University.
1993-2001	Professor, Department of Biochemistry and Molecular Biology, OGI School of Science & Engineering, Oregon Health & Science University (Department Head, 1993-1996).
1980-1981	Visiting Professor, Laboratory of Physical Chemistry, The Norwegian Institute of Technology, Trondheim, Norway.
1971-1993	Associate Professor, then Professor, Department of Pharmacology, Medical College of Ohio.
1965-1971	Instructor, then Assistant Professor, Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine.
1961-1965	NIH Postdoctoral Fellow, Department of Pharmacology & Experimental Therapeutics (G.H. Mudge and I.M. Weiner) and Department of Biophysics (M. Larrabee), The Johns Hopkins University.
1958-1961	Predoctoral Fellow, Department of Pharmacology & Experimental Therapeutics (G.H. Mudge and I.M. Weiner), The Johns Hopkins University.

Dissertation

THE K+/H+ ANTIPORTER OF MITOCHONDRIA AND ITS ROLE IN VOLUME HOMEOSTASIS, Dr. techn. thesis - April, 1987 Division of Physical Chemistry Norwegian Institute of Technology N-7034 Trondheim - NTH Norway.

Refereed Publications

Chapters

1.   K. D. Garlid (1976) Free and bound water in mitochondria: two distinct aqueous phases with different solution properties. In: L'Eau et les Systemes Biologiques (A. J. Berteaud and A. Alfsen, eds.), Colloques Internationaux du C.N.R.S., Paris, pp. 317-321.

2.   K. D. Garlid (1978) Overview of our understanding of intracellular water in hydrated cells. In: Dry Biological Systems (J. H. Crowe and J. S. Clegg, eds.), Academic Press, New York, pp. 3-19.

3.   K. D. Garlid (1988) Mitochondrial volume control. In: Integration of Mitochondrial Function (J. J. Lemasters, C. R. Hackenbrock, R. G. Thurman, and H. V. Westerhoff, eds.), Plenum Publishing Corp., New York, pp. 259-278.

4.   S. Nath and K. D. Garlid (1988) Lithium ion is a competitive inhibitor of the Na+ selective Na+/H+ antiporter from mitochondria. In: Integration of Mitochondrial Function (J. J. Lemasters, C. R. Hackenbrock, R. G. Thurman, and H. V. Westerhoff, eds.), Plenum Publishing Corp., New York, pp. 355-362.

5.   N. Siliprandi, D. Siliprandi, A. Toninello, and K. Garlid (1988) Polyamine transport in liver mitochondria. In: Molecular Basis of Biomembrane Transport (F. Palmieri and E. Quagliariello, eds.), Elsevier Science Publishers, Amsterdam, pp. 155-162.

6.   K. D. Garlid, C. S. Semrad, and V. Zinchenko (1992) Does redox slip contribute significantly to mitochondrial respiration? In: Modern Trends in Biothermokinetics (S. Schuster, M. Rigoulet, R. Ouhabi, and J.-P. Mazat, eds.), Plenum Press, New York, pp. 287-293.

7.   K. D. Garlid (1998) Physiology of Mitochondria. In: Cell Physiology Source Book, 2nd Edition (N. Sperelakis, ed.) Academic Press, San Diego, pp. 111-119.

8.   K. D. Garlid (2001) The physiology of mitochondria. In: The Cell Physiology Source Book, 3rd Edition (N. Sperelakis, ed.), Academic Press, San Diego, pp. 139-151.

9.   M. N. Laclau, P. Dos Santos, L. Tariosse, S. Boudina, and K. D. Garlid (2002) The essential role of the mitochondrial ATP_sensitive potassium channel in cardiac inotropy. Advances in Recent Cardiovascular Research, Proceedings, Monduzzi Editore, Bologna, p. 47-54.

Articles

   1.   I. M. Weiner, K. D. Garlid, D. Sapir, and G. H. Mudge (1959) The effects of dimercaprol (BAL) on the renal excretion of mercurials. J. Pharmacol. Exp. Ther. 127, 325-331.

   2.   G. H. Mudge, K. D. Garlid, and I. M. Weiner (1962) Renal tubular secretion of o-acetylaminohippurate. Am. J. Physiol. 203, 881-885.

   3.   S. J. Lloyd, K. D. Garlid, R. C. Reba, and A. E. Seeds (1969) Permeability of different layers of the human placenta to isotopic water. J. Appl. Physiol. 26, 274-276.

   4.   J. L. Gamble, Jr. and K. D. Garlid (1970) The permeability of the mitochondrial inner membrane to sucrose. Biochim. Biophys. Acta 211, 223-232.

   5.   A. E. Seeds, J. J. Schruefer, J. A. Reinhardt, and K. D. Garlid (1973) Diffusion mechanisms across human placental tissue. Gynecol. Invest. 4, 31-37.

   6.   J. J. Schruefer, D. R. Harbuchak, K. D. Garlid, and A. E. Seeds, Jr. (1975) Non-ionic diffusion of pentobarbital across human chorion laeve. Biol. Neonate 27, 251-258.

   7.   K. D. Garlid (1978) Unmasking the mitochondrial K/H exchanger: swelling-induced K+-loss. Biochem. Biophys. Res. Comm. 83, 1450-1455.

   8.   K. D. Garlid (1979) Unmasking the mitochondrial K/H exchanger: tetraethylammonium-induced K+-loss. Biochem. Biophys. Res. Comm. 87, 842-847.

   9.   K. D. Garlid (1979) Aqueous phase structure in cells and organelles. In: Cell-Associated Water (W. Drost-Hansen and J. S. Clegg, eds.), Academic Press, New York, pp. 293-361.

   10.   R. S. Dordick, G. P. Brierley, and K. D. Garlid (1980) On the mechanism of A23187-induced potassium efflux in rat liver mitochondria. J. Biol. Chem. 255, 10299-10305.

   11.   G.-Y. Shi, D. W. Jung, K. D. Garlid, and G. P. Brierley (1980) Induction of respiration-dependent net efflux of K+ from heart mitochondria by depletion of endogenous divalent cations. J. Biol. Chem. 255, 10306-10311.

   12.   K. D. Garlid (1980) On the mechanism of regulation of the mitochondrial K+/H+ exchanger. J. Biol. Chem. 255, 11273-11279.

   13.   R. A. Nakashima and K. D. Garlid (1982) Quinine inhibition of Na+ and K+ transport provides evidence for two cation/H+ exchangers in rat liver mitochondria. J. Biol. Chem. 257, 9252-9254.

   14.   R. A. Nakashima, R. S. Dordick, and K. D. Garlid (1982) On the relative roles of Ca2+ and Mg2+ in regulating the endogenous K+/H+ exchanger of rat liver mitochondria. J. Biol. Chem. 257, 12540-12545.

   15.   K. D. Garlid and R. A. Nakashima (1983) Studies on the mechanism of uncoupling by amine local anesthetics. Evidence for mitochondrial proton transport mediated by lipophilic ion pairs. J. Biol. Chem. 258, 7974-7980.

   16.   W. H. Martin, A. D. Beavis, and K. D. Garlid (1984) Identification of an 82,000-dalton protein responsible for K+/H+ antiport in rat liver mitochondria. J. Biol. Chem. 259, 2062-2065.

   17.   A. Lyslo, S. Kvernes, K. Garlid, and S. K. Ratkje (1985) Ionic transport across corneal endothelium. Acta Ophthalmol. 63, 116-125.

   18.   A. D. Beavis, R. D. Brannan, and K. D. Garlid (1985) Swelling and contraction of the mitochondrial matrix. I. A structural interpretation of the relationship between light scattering and matrix volume. J. Biol. Chem. 260, 13424-13433.

   19.   K. D. Garlid and A. D. Beavis (1985) Swelling and contraction of the mitochondrial matrix. II. Quantitative application of the light scattering technique to solute transport across the inner membrane. J. Biol. Chem. 260, 13434-13441.

   20.   K. D. Garlid and A. D. Beavis (1986) Evidence for the existence of an inner membrane anion channel in mitochondria. Biochim. Biophys. Acta 853, 187-204.

   21.   K. D. Garlid, D. J. DiResta, A. D. Beavis, and W. H. Martin (1986) On the mechanism by which dicyclohexylcarbodiimide and quinine inhibit K+ transport in rat liver mitochondria. J. Biol. Chem. 261, 1529-1535.

   22.   W. H. Martin, D. J. DiResta, and K. D. Garlid (1986) Kinetics of inhibition and binding of dicyclohexylcarbodiimide to the 82,000-dalton mitochondrial K+/H+ antiporter. J. Biol. Chem. 261, 12300-12305.

   23.   D. J. DiResta, K. P. Kutschke, M. D. Hottois, and K. D. Garlid (1986) K+-H+ exchange and volume homeostasis in brown adipose tissue mitochondria. Am. J. Physiol. 251, R787-R793.

   24.   A. D. Beavis and K. D. Garlid (1987) The mitochondrial inner membrane anion channel. Regulation by divalent cations and protons. J. Biol. Chem. 262, 15085-15093.

   25.   K. D. Garlid (1988) Sodium/proton antiporters in the mitochondrial inner membrane. Adv. Exp. Med. Biol. 232, 37-46.

   26.   A. D. Beavis and K. D. Garlid (1988) Inhibition of the mitochondrial inner membrane anion channel by dicyclohexylcarbodiimide. Evidence for a specific transport pathway. J. Biol. Chem. 263, 7574-7580.

   27.   A. Toninello, G. Miotto, D. Siliprandi, N. Siliprandi, and K. Garlid (1988) On the mechanism of spermine transport in liver mitochondria. J. Biol. Chem. 263, 19407-19411.

   28.   S. S. Kakar, F. Mahdi, X. Q. Li, and K. D. Garlid (1989) Reconstitution of the mitochondrial non-selective Na+/H+ (K+/H+) antiporter into proteoliposomes. J. Biol. Chem. 264, 5846-5851.

   29.   K. D. Garlid, A. D. Beavis, and S. K. Ratkje (1989) On the nature of ion leaks in energy-transducing membranes. Biochim. Biophys. Acta 976, 109-120.

   30.   P. Jezek, A. D. Beavis, D. J. DiResta, R. N. Cousino, and K. D. Garlid (1989) Evidence for two distinct chloride uniport pathways in brown adipose tissue mitochondria. Am. J. Physiol. 257, C1142-C1148.

   31.   A. D. Beavis and K. D. Garlid (1990) Evidence for the allosteric regulation of the mitochondrial K+/H+ antiporter by matrix protons. J. Biol. Chem. 265, 2538-2545.

   32.   S. M. Periyasamy, S. S. Kakar, K. D. Garlid, and A. Askari (1990) Ion specificity of cardiac sarcolemmal Na+/H+ antiporter. J. Biol. Chem. 265, 6035-6041.

   33.   P. Jezek, F. Mahdi, and K. D. Garlid (1990) Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter. Quantitation of K+ transport with the novel fluorescent probe, PBFI. J. Biol. Chem. 265, 10522-10526.

   34.   K. D. Garlid (1990) New insights into mechanisms of anion uniport through the uncoupling protein of brown adipose tissue mitochondria. Biochim. Biophys. Acta 1018, 151-154.

   35.   X. Q. Li, M. G. Hegazy, F. Mahdi, P. Jezek, R. D. Lane, and K. D. Garlid (1990) Purification of a reconstitutively active K+/H+ antiporter from rat liver mitochondria. J. Biol. Chem. 265, 15316-15322.

   36.   P. Jezek, D. E. Orosz, and K. D. Garlid (1990) Reconstitution of the uncoupling protein of brown adipose tissue mitochondria. Demonstration of GDP-sensitive halide anion uniport. J. Biol. Chem. 265, 19296-19302.

   37.   P. Jezek, and K. D. Garlid (1990) New substrates and competitive inhibitors of the Cl- translocating pathway of the uncoupling protein of brown adipose tissue mitochondria. J. Biol. Chem. 265, 19303-19311.

   38.   K. D. Garlid, Z. Shariat-Madar, S. Nath, and P. Jezek (1991) Reconstitution and partial purification of the Na+-selective Na+/H+ antiporter of beef heart mitochondria. J. Biol. Chem. 266, 6518-6523.

   39.   D. L. Murdza-Inglis, H. V. Patel, K. B. Freeman, P. Jezek, D. E. Orosz, and K. D. Garlid (1991) Functional reconstitution of rat uncoupling protein following its high level expression in yeast. J. Biol. Chem. 266, 11871-11875.

   40.   W. Li, Z. Shariat-Madar, M. Powers, X. Sun, R. D. Lane, and K. D. Garlid (1992) Reconstitution, identification, purification, and immunological characterization of the 110-kDa Na+/Ca2+ antiporter from beef heart mitochondria. J. Biol. Chem. 267, 17983-17989.

   41.   A. Toninello, L. Dalla Via, D. Siliprandi, and K. D. Garlid (1992) Evidence that spermine, spermidine, and putrescine are transported electrophoretically in mitochondria by a specific polyamine uniporter. J. Biol. Chem. 267, 18393-18397.

   42.   X. Sun and K. D. Garlid (1992) On the mechanism by which bupivacaine conducts protons across the membranes of mitochondria and liposomes. J. Biol. Chem. 267, 19147-19154.

   43.   P. Paucek, G. Mironova, F. Mahdi, A. D. Beavis, G. Woldegiorgis, and K. D. Garlid (1992) Reconstitution and partial purification of the glibenclamide-sensitive, ATP-dependent K+ channel from rat liver and beef heart mitochondria. J. Biol. Chem. 267, 26062-26069.

   44.   A. D. Beavis, Y. Lu, and K. D. Garlid (1993) On the regulation of K+ uniport in intact mitochondria by adenine nucleotides and nucleotide analogs. J. Biol. Chem. 268, 997-1004.

   45.   D. E. Orosz, and K. D. Garlid (1993) A sensitive new fluorescence assay for measuring proton transport across liposomal membranes. Anal. Biochem. 210, 7-15.

   46.   A. A. Welihinda, R. J. Trumbly, K. D. Garlid, and A. D. Beavis (1993) On the regulation of Na+/H+ and K+/H+ antiport in yeast mitochondria: evidence for the absence of an Na+-selective Na+/H+ antiporter. Biochim. Biophys. Acta, 1144, 367-373.

   47.   D. L. Murdza-Inglis, M. Modriansky, H. V. Patel, G. Woldegiorgis, K. B. Freeman, and K. D. Garlid (1994) A single mutation in uncoupling protein of rat brown adipose tissue mitochondria abolishes GDP sensitivity of H+ transport. J. Biol. Chem. 269, 7435-7438.

   48.   X. Sun, J. R. Wong, K. Song, J. Hu, K. D. Garlid, and L. B. Chen (1994) AA1, a newly synthesized monovalent lipophilic cation, expresses potent in vivo antitumor activity. Cancer Res. 54, 1465-1471.

   49.   S. M. Periyasamy, P. Paucek, K. D. Garlid, and A. Askari (1994) Reconstitution and partial purification of cardiac sarcolemmal Na+/H+ (K+/H+) antiporter. Arch. Biochem. Biophys. 313, 184-188.

   50.   P. Jezek, D. E. Orosz, M. Modriansky, and K. D. Garlid (1994) Transport of anions and protons by the mitochondrial uncoupling protein and its regulation by nucleotides and fatty acids. A new look at old hypotheses. J. Biol. Chem. 269, 26184-26190.

   51.   K. D. Garlid (1994) Mitochondrial cation transport: A progress report. J. Bioenerg. Biomembr. 26, 537-542.

   52.   K. D. Garlid, X. Sun, P. Paucek, and G. Woldegiorgis (1995) Mitochondrial cation transport systems. Methods Enzymol. 260, 331-348.

   53.   P. Jezek, M. Bauer, P. D. Vogel, K. D. Garlid, and W. E. Trommer (1995) Fatty acid binding to the mitochondrial uncoupling protein. Bull. Magn. Reson. 17, 126-129.

   54.   K. D. Garlid, D. E. Orosz, M. Modriansky, S. Vassanelli, and P. Jezek (1996) On the mechanism of fatty acid-induced proton transport by mitochondrial uncoupling protein. J. Biol. Chem. 271, 2615-2620.

   55.   P. Jezek, J. Hanus, C. Semrad, and K. D. Garlid (1996) Photoactivated azido fatty acid irreversibly inhibits anion and proton transport through the mitochondrial uncoupling protein. J. Biol. Chem. 271, 6199-6205.

   56.   K. D. Garlid, P. Paucek, V. Yarov-Yarovoy, X. Sun, and P. A. Schindler (1996) The mitochondrial KATP channel as a receptor for potassium channel openers. J. Biol. Chem. 271, 8796-8799.

   57.   K. D. Garlid (1996) Cation transport in mitochondria B the potassium cycle. Biochim. Biophys. Acta 1275, 123-126.

   58.   P. Paucek, V. Yarov-Yarovoy, X. Sun, and K. D. Garlid (1996) Inhibition of the mitochondrial KATP channel by long-chain acyl-CoA esters and activation by guanine nucleotides. J. Biol. Chem. 271, 32084-32088.

   59.   P. Jezek, M. Modriansky, and K. D. Garlid (1997) Inactive fatty acids are unable to flip-flop across the lipid bilayer. FEBS Lett. 408, 161-165.

   60.   P. Jezek, M. Modriansky, and K. D. Garlid (1997) A structure-function study of fatty acid interaction with mitochondrial uncoupling protein. FEBS Lett. 408, 166-170.

   61.   V. Yarov-Yarovoy, P. Paucek, M. Jaburek, and K. D. Garlid (1997) The nucleotide regulatory sites on the mitochondrial KATP channel face the cytosol. Biochim. Biophys. Acta, 1321, 128-136.

   62.   M. Modriansky, D. L. Murdza-Inglis, H. V. Patel, K. B. Freeman, and K. D. Garlid (1997) Identification by site-directed mutagenesis of three arginines in uncoupling protein that are essential for nucleotide binding and inhibition. J. Biol. Chem. 272, 24759-24762.

   63.   K. D. Garlid, P. Paucek, V. Yarov-Yarovoy, H. N. Murray, R. B. Darbenzio, A. J. D'Alonzo, N. J. Lodge, M. A. Smith, and G. J. Grover (1997) Cardioprotective effect of diazoxide and its interaction with mitochondrial ATP-sensitive K+ channels. Possible mechanism of cardioprotection. Circ. Res. 81, 1072-1082.

   64.    M. Jaburek, V. Yarov-Yarovoy, P. Paucek, and K. D. Garlid (1998) State-dependent inhibition of the mitochondrial KATP channel by glyburide and 5-hydroxydecanoate. J. Biol. Chem. 273, 13578-13582.

   65.   P. Jezek, H. Engstova, M. Zackova, A. E. Vercesi, A. D. T. Costa, P. Arruda, and K. D. Garlid (1998) Fatty acid cycling mechanism and mitochondrial uncoupling proteins. Biochim. Biophys. Acta 1365, 319-327.

   66.   K. D. Garlid, M. Jaburek, and P. Jezek (1998) The mechanism of proton transport mediated by mitochondrial uncoupling proteins. FEBS Lett. 438, 10-14.

   67.   P. Jezek and K. D. Garlid (1998) Mammalian mitochondrial uncoupling proteins. Int. J. Biochem. Cell Biol. 30, 1163-1168. Review.

   68.   P. Jezek, M. Zackova, Z. Rehakova, M. Ruzicka, J. Borecky, E. Skobisova, J. Brucknerova, K. D. Garlid, R. E. Gimeno, and L. A. Targaglia (1999) Existence of uncoupling protein-2 antigen in isolated mitochondria from various tissues. FEBS Lett. 455, 79-82.

   69.   M. Jaburek, M. Varecha, R. E. Gimeno, M. Dembski, P. Jezek, M. Zhang, P. Burn, L. A. Tartaglia, and K. D. Garlid (1999) Transport function and regulation of mitochondrial uncoupling proteins 2 and 3. J. Biol. Chem. 274, 26003-26007.

   70.   B. J. Pomerantz, J. C. Cleveland, Jr., K. D. Garlid, A. Banerjee, and A. H. Harken (1999) Adenosine-mediated cardiac preconditioning extends beyond opening of the mitochondrial KATP channel in human myocardium. Surg. Forum 50, 94-96.

   71.   K. D. Garlid (2000) The state of water in biological systems. Int. Rev. Cytol. 192, 281-302.

   72.   G. J. Grover and K. D. Garlid (2000) ATP-sensitive potassium channels: a review of their cardioprotective pharmacology. J. Mol. Cell. Cardiol. 32, 677-695.

   73.   P. E. Puddu, K. D. Garlid, F. Monti, K. Iwashiro, S. Picard, A. A. Dawodu, A. Criniti, G. Ruvulo, and P. P. Campa (2000) Bimakalim: a promising KATP channel activating agent. Cardiovasc. Drug. Rev. 18, 25-46.

   74.   K. D. Garlid, M. Jaburek, P. Jezek, and M. Varecha (2000) How do uncoupling proteins uncouple? Biochim. Biophys. Acta 1459, 383-389.

   75.   K. D. Garlid (2000) Opening mitochondrial KATP in the heartCwhat happens, and what does not happen. Basic. Res. Cardiol. 95, 275-279.

   76.   A. J. Kowaltowski, S. Seetharaman, P. Paucek, and K. D. Garlid (2001) Bioenergetic consequences of opening the ATP-sensitive K+ channel of heart mitochondria. Am. J. Physiol. Heart Circ. Physiol. 280, H649-H657.

   77.   M. N. Laclau, S. Boudina, J. B. Thambo, L. Tariosse, G. Gouverneur, S. Bonoron-Adele, V. A. Saks, K. D. Garlid, and P. Dos Santos (2001) Cardioprotection by ischemic preconditioning preserves mitochondrial function and functional coupling between adenine nucleotide translocase and creatine kinase. J. Mol. Cell. Cardiol. 33, 947-956.

   78.   G. J. Grover, A. J. D'Alonzo, K. D. Garlid, R. Bajgar, N. J. Lodge, P. G. Sleph, R. B. Darbenzio, T. A. Hess, M. A. Smith, P. Paucek, and K. S. Atwal (2001) Pharmacologic characterization of BMS-191095, a mitochondrial KATP opener with no peripheral vasodilator or cardiac action potential shortening activity. J. Pharmacol. Exp. Ther. 297, 1184-1192.

   79.   M. Jaburek, M. Vaoecha, P. Jezek, and K. D. Garlid (2001) Alkylsulfonates as probes of uncoupling protein transport mechanism. Ion pair transport demonstrates that direct H+ translocation by UCP1 is not necessary for uncoupling. J. Biol. Chem. 276, 31897-31905.

   80.   R. Bajgar, S. Seetharaman, A. J. Kowaltowski, K. D. Garlid, and P. Paucek (2001) Identification and properties of a novel intracellular (mitochondrial) ATP-sensitive potassium channel in brain. J. Biol. Chem. 276, 33369-33374.

   81.   K. D. Garlid, M. Jaburek, and P. Jezek (2001) Mechanism of uncoupling protein action. Biochem. Soc. Trans. 29, 803-806.

   82.   K. D. Garlid, and P. Paucek (2001) The mitochondrial potassium cycle. IUBMB Life 52, 153-158.

   83.   P. Dos Santos, A. J. Kowaltowski, M. N. Laclau, S. Seetharaman, P. Paucek, S. Boudina, J.-B. Thambo, L. Tariosse, and K. D. Garlid (2002) Mechanisms by which opening the mitochondrial ATP-sensitive potassium channel protects the ischemic heart. Am. J. Physiol. Heart Circ. Physiol. 283, H284-95.

   84.   M. Krenz, O. Oldenburg, H. Wimpee, M. V. Cohen, K. D. Garlid, S. D. Critz, J. M. Downey, and J. N. Benoit (2002) Opening of ATP-sensitive potassium channels causes generation of free radicals in vascular smooth muscle cells. Basic Res. Cardiol. 97, 365-375.

       85.   J. Tian, J. Liu, J. I. Shapiro, K. D. Garlid, and Z. Xie (2002) Involvement of Mitogen-activated Protein Kinases and Reactive Oxygen Species in the Inotropic Action of Ouabain on Cardiac Myocytes. A Potential Role for Mitochondrial KATP Channels. Mol Cell Biochem 242, 181-187.

   86.   K. D. Garlid, P. Dos Santos, Z. Xie, and P. Paucek (2003) Mitochondrial potassium transport: the role of the mitochondrial ATP-sensitive K+channel in cardiac function and cardioprotection. Biochim Biophys Acta, Submitted and in revision.

   87.   K. D. Garlid, and P. Paucek (2003) Mitochondrial potassium transport: the K+ cycle. Biochim Biophys Acta, Submitted and in revision.

   88.   O. Oldenburg, X-M. Yang, K. D. Garlid, M. V. Cohen, G. J. Grover, and J. M. Downey (2003) P1075 Opens Mitochondrial KATP Channels and Generates Reactive Oxygen Species Resulting in Cardioprotection of Rabbit Hearts. J. Mol. Cell. Cardiol. Submitted and in review.

Abstracts (1999- Present)

P. Paucek, R. Bajgar, and K. D.Garlid (1999) Regulatory Sites of MitoKATP are on MitoSUR. Biophys. J. 76, A328.

R. Bajgar, P. Paucek, G. Fiskum, and K. D. Garlid (2000) Identification and functional reconstitution of brain mitochondrial mitoKATP. Biophys. J. 78, 139A.

P. Paucek, R. Bajgar, A. J. Kowaltowski, S. Seetharaman, and K. D. Garlid (2000) The mitochondrial ATP-sensitive K+ channel (mitoKATP). EBEC Short Rep. 11, 96.

K. D. Garlid, A. J. Kowaltowski, S. Seetharaman, and P. Paucek (2000) The mitochondrial KATP channelCits role in cardiac function and in prevention of ischemia-reperfusion injury. Eur. J. Med. Res. 5, 17.

P. Paucek, K. D. Garlid, A. J. D'Alonzo, P. G. Sleph, R. B. Darbenzio, T. A. Hess, K. S. Atwal, and G. J. Grover (2000) BMS-191095, a selective mitochondrial KATP opener. J. Mol. Cell. Cardiol. 32, A53.

A. Banerjee, B. J. Pomeranz, T. Robinson, T. D. Morrell, A. H. Harken, K. D. Garlid, and C. B. Cairns (2000) Mitochondrial KATP channel opening confers protection against ischemia-reperfusion injury in human myocardium by enhanced oxygen consumption during reperfusion. 73rd American Heart Association Scientific Sessions.

A. J. Kowaltowski, S. Seetharaman, P. Paucek, and K. D. Garlid (2001) The mitochondrial ATP-sensitive K+ channel regulated oxidative phosphorylation and ATP hydrolysis. Biophys. J. 80, 498a.

P. Paucek, R. Bajgar, S. Seetharaman, G. Grover, M. A. Smith, P. G. Sleph, and K. D. Garlid (2001) The cardioprotective P-1075 targets mitoKATP in heart mitochondria. J. Mol. Cell. Cardiol. 33, A91.

S. Seetharaman, V. Zinchenko P. Paucek, and K. D. Garlid (2002) Volume activation of mitochondrial electron transport. Biophys. J. 82, 109A.

P. Paucek, M. Jaburek, P. Jezek, J. Jezek, and K. D. Garlid (2002) Purification of brain mitochondrial Na+/Ca2+ antiporter. Biophys. J. 82, 109A.

P. Paucek, L. Dolgacova, and K. D. Garlid (2002) Purification and reconstitution of KATP channel (pmitoKATP) of plant mitochondria. Biophys. J. 82, 109A.

P. Paucek, E. Crucerescu, and K. D. Garlid (2002) Purification of mitoKATP subunits for amino acid sequencing. Biophys. J. 82, 109A.

Tian, J., Liu, J., Garlid, K.D. and Xie, Z. (2002) Signal-transducing function of Na/K-ATPase is involved in regulation of contractility by ouabain in cardiac myocytes, Biophys. J. In press.

P. Dos Santos, A. J. Kowaltowski, M. N. Laclau, S. Seetharaman, P. Paucek, S. Boudina, J. B. Thambo, L. Tariosse, and K. D. Garlid (2002) Mechanism by which opening the mitochondrial ATP-sensitive potassium channel protects the ischemic heart. J Mol Cell Cardiol 34, A21, 2002.

M. Laclau, P. Dos Santos, L. Tariosse, S. Boudina, and K. D. Garlid (2002) Opening of heart mitochondrial ATP-sensitive potassium channel is mandatory to respond to calcium-induced positive inotropic stress. J Mol Cell Cardiol 34, A37, 2002.

Presentations at Professional Meetings (1999- Present)

"Transport Function and Regulation of Uncoupling Proteins 2 and 3," Biophysical Society Meeting, Baltimore, MD, February 13, 1999. (Session Subgroup Chair)

The Third Conference on ABC and Ion Channels: From Gene to Disease," Kyoto, Japan, March 12-13, 1999.

Biochemistry and Molecular Biology '99, San Francisco, CA, May 20, 1999.

"Mammalian Uncoupling Proteins: Transport Mechanisms and Regulation," Brazilian Biochemistry Meeting, Caxambu, Brazil, May 21 - June 2, 1999.

"Role of Mitochondrial KATP Channel in Cell Death and Cell Survival," 58th Scientific Sessions, American Diabetes Association, San Diego, CA, 6/20/99. (Session Chair: Mitochondrial Function in Diabetes, 6/19/99)

"Mitochondrial KATP Channels," XXIst Congress of the European Society of Cardiology, Barcelona, Spain, 8/29/99.

"The Function of Mitochondrial KATP Channel in Normal and Ischemic Heart," Sixth International Symposium on Adenosine, Cardioprotection and Its Clinical Application, Atlanta, GA, 11/6/99.

"Mitochondrial KATP Channels: Physiology and Pathophysiology." Keystone Symposium on Mitochondrial Dysfunction in Pathogenesis, Santa Fe, NM, 01/15-20/00.

"The Mitochondrial KATP ChannelCIts Role in Cardiac Function and in Prevention of Ischemia-reperfusion Injury." 2nd Colloquium on Mitochondria and Myopathies, Halle (Saale), Germany, 4/1/00.

"Bioenergetic Consequences of Opening and Closing MitoKATP." Symposium on the Developing Heart, Prague, Czech Republic, 5/5/00.

"Identification and Functional Reconstitution of the ATP-sensitive Potassium Channel of Brain Mitochondria." Fernstrom Foundation Symposium, The Role of Mitochondria in Apoptosis and Neurodegeneration, Lund, Sweden, 6/2-6/00.

"The Mitochondrial ATP-sensitive K+ Channel-Its Role in Normal and Ischemic Heart." International Society for Heart Research, Louisville, KY, 6/14-18/00.

"How Do Uncoupling Proteins Uncouple?" European Bioenergetics Congress, Brighton, UK, 9/9-13/00.

"The Role of the Mitochondrial ATP-Sensitive K+ Channel in Heart." International Meeting of the International Society for Heart Research, Winnipeg, Canada, 7/6-12/01.

"Mechanism of Uncoupling Protein Action." Biochemical Society (UK) Symposium on Signaling Homeostasis, Dublin, Ireland, 7/12-14/01.

"The Role of the Mitochondrial ATP-Sensitive K+ Channel in Ischemic Preconditioning." American Heart Association Conference on Molecular, Inegrative, and Clinical Approaches to Myocardial Ischemia, Seattle, WA, 8/9-11/01.

“The essential role of the mitochondrial ATP_sensitive potassium channel in cardiac inotropy.” Advances in Recent Cardiovascular Research, Monduzzi Editore, Bologna, Italy, 2002.

"How does opening the mitochondrial ATP-sensitive K+ channel protect the heart?" International Society for Heart Research, Szeged, Hungary, July 1-6, 2002.

"Function of Uncoupling Proteins as Deduced from Reconstitution Studies". Kern Aspen Lipid Conference, Aug 17-20, 2002.

"Mitochondrial physiology". Biothermokinetics Congress, Arcachon, France, Sept 7 - 10, 2002.

"Implication of mitochondrial K-ATP channels in physiology of the heart and cardioprotection against ischemia-reperfusion: Overview." European Bioenergetics Congress, Arcachon, France, Sept 10 - 15.

"Potassium channels in heart". Session moderator, American Heart Association, Nov 11-15, 2002.

Invited Seminars

Fall 1999 Biochemistry Seminar, University of California-San Diego, La Jolla, CA, 9/30/99.

"Pharmacology of the Mitochondrial ATP-sensitive K+ Channel." Aventis Pharmaceutical Company, Frankfurt, Germany, 3/29/00.

"Biochemistry of the Mitochondrial KATP Channel." University of Constanz, Germany, 4/3/00.

"The Mitochondrial ATP-sensitive K+ Channel." ETH-Zurich, Switzerland, 4/5/00.

"The Mitochondrial KATP Channel and Its Role in Heart." Inaugural Lecture, Trinity College, Dublin, Ireland, 4/18/00.

"The Mechanism of Uncoupling by Uncoupling Proteins." Institute of Physiology, Czech Academy of Sciences, Prague, 5/19/00.

"The Role of the Mitochondrial KATP Channel in Heart." Cardiovascular Research Unit, University of Bordeaux, France, 5/23/00.

"The Roles of the Mitochondrial KATP Channel in Heart Function and in Ischemic Protection." Centre for Cardiovascular Biology and Medicine, King's College London, UK, 9/14/00.

"The Roles of the Mitochondrial KATP Channel in Heart Function and in Ischemic Protection." Hatter Institute and Centre for Cardiology, University College Hospital and Medical School, London, 9/15/00.

"How Does Opening the Mitochondrial ATP-Sensitive K+ Channel Protect the Heart?" Department of Physiology, University of Texas Southwestern Medical School, Dallas, 9/10/01.

"The Role of the Mitochondrial ATP-Sensitive K+ Channel in the Heart." The Medical College of Ohio, Toledo, 11/8/01.

"The Mitochondrial ATP-Sensitive K+ ChannelCMechanisms of Cardioprotection." INSERM, Université Paris-Sud, Paris-Santenay, France, 11/29/01.

"The Role of the Mitochondrial KATP Channel in Heart." INSERM, University of Bordeaux School of Medicine, Bordeaux, France, 12/13/01.

"The pharmacology of cardiac mitoKATP channels". Aventis Corporation, Frankfurt, Germay, Sept 5, 2002.

"The role of the mitochondrial ATP-sensitive K+ channel in cardioprotection". Univ. of Essen, Essen, Germany, Sept 17, 2002.

Friday, 9/20, I will give a seminar, "The mitochondrial ATP-sensitive potassium channel." University of Tromso, Tromso, Norway, Sept 20, 2002.

"The mitochondrial ATP-sensitive potassium channel and its role in heart." University of Trondheim, Trondheim, Norway, Sept 23, 2002.
"Physiological roles of the mitochondrial ATP-sensitive K+ channel (mitoK ATP ) in heart." University of Chicago, Nov 8, 2002.

Scholarly Works in Progress

Manuscripts in Preparation or Revision

P. Paucek, R. Bajgar, G. Grover, H. Englert, and K. D. Garlid. Pharmacology of mitochondrial ATP-sensitive potassium channel, mitoKATP. I. Potassium channel openers. In preparation.

R. Bajgar, P. Paucek, G. Grover, and K. D. Garlid. Pharmacological profile of mitochondrial ATP-sensitive potassium channel. II. Potassium channel blockers. In preparation.

M. Jaburek, P. Paucek, and K. D. Garlid. Kinetics and ion specificity of Na+/Ca2+ exchange mediated by the reconstituted beef heart mitochondrial Na+/Ca2+ antiporter. In preparation.

G. D. Mironova, A. E. Negoda, S. M. Grigoriev, Y. Y. Skarga, R. Bajgar, A. J. Kowaltowski, P. Paucek, A. Zapodeanu, and K. D. Garlid. Nucleotide regulation of the mitochondrial ATP-sensitive K+ channelCmitoKATP is an intracellular UDP receptor. In preparation.

Laclau, M.N., Dos Santos, P., Tariosse, L., Boudina, S. and Garlid, K.D. (2002) The essential role of the mitochondrial ATP-sensitive potassium channel in cardiac inotropy. In preparation.

Honors, Grants, and Fellowships

Honors and Fellowships

Fogarty Senior International Fellow, NIH, Department of Physical Chemistry, Norwegian Institute of Technology, Trondheim, 1980-1981.
Senior Visiting Scientist Award, Royal Norwegian Council for Scientific Research, 1980-1981.

Distinguished Visiting Professor in Biology, University of Miami, Fall Semester, 1983.

International Exchange Fellow, National Academy of Sciences (U.S.) to Czechoslovakia Academy of Sciences, Prague, May-June, 1984.

Honorary Alumnus, Glynn Research Foundation, Bodmin, Cornwall, U.K..

Pametni Medaile (Commemorative medal) for distinguished service to academia, from the board of directors of Palacky University, Czech Republic, December 11, 1999.

Elected Fellow, Academy of International Institute of Cardiovascular Sciences.

Current Grants

"Function and Regulation of Uncoupling Proteins 2 and 3"
PI: K. D. Garlid
NIH RO1 DK56273 (Years 1-4)
8/1/99 - 7/31/03, $1,189,564

Support for Mitochondrial Research
Aventis Pharma Deutschland GmbH
PI: K. D. Garlid
3/1/00 - 3/9/05, $20,000

"Regulation of Novel Mitochondrial Uncoupling Proteins"
PI: K. D. Garlid
NIH R03 TW01487-01A2
7/1/02 - 6/30/05, $112,640
Funding for international collaborator Petr Jezek

"Role of the Mitochondrial ATP-Sensitive K+ Channel in Heart"
PI: K. D. Garlid
NIH R01 HL67842-01A1
7/1/02 - 6/30/07, $2,050,000

"Control Mechanisms of Cardiac Proteins and Enzymes:
Project IV: Cardiac Mitochondrial KATP Channel and Ouabain Signaling"
PI: Amir Askara ; Project leader: K. D. Garlid
NIH PO1 HL36573-06
3/1/03 - 2/28/08, $2,250,067 (Project IV component)

Pending Grant Applications

"Function and Regulation of Uncoupling Proteins 2 and 3"
PI: K. D. Garlid
NIH RO1 DK56273 (Years 5-8)
8/1/03 - 7/31/07, $1,189,564

Past Grants

"Ion Transport Mechanisms in Mitochondria"
PI: K. D. Garlid
NIH, RO1 GM31086 (Years 13-16)
7/1/84 - 11/30/00, $150,000 per year

"Structure/Function of Cardiac K+/H+ and Na+/H+ Exchangers"
PI: K. D. Garlid
NIH RO1 HL43814-02,
1990-1995, $180,000 per year

"Control Mechanisms of Cardiac Proteins and Enzymes" (Program Project Grant)
Project III: "Cardiac Cation Exchange Carriers"
PI: Amir Askara ; Project leader: K. D. Garlid
NIH PO1 HL36573-06
1991-1996, $150,000 per year

"Uncoupling Protein 2 in Obesity"
PI: K. D. Garlid
Millennium Pharmaceuticals (Boston)
10/1/98 - 9/30/99 (6/30/00), $40,500

"Structure-Function of the Mitochondrial KATP Channel"
PI: K. D. Garlid
NIH, RO1 GM55324 (Years 1-4)
4/1/98 - 3/31/02, $1,021,817

"Structure-Function of Mitochondrial KIR"
PI: K. D. Garlid
NIH-FIRCA RO3 TWO1116 (Years 1-3)
9/30/99 - 9/29/02, $117,840
Funding for international collaborator Galina Mironova

Other Teaching, Mentoring and Curricular Achievements

Courses Taught

Medical Pharmacology:	Anesthetics, Antidepressants, Antipsychotics
Graduate courses:	Bioenergetics, Membrane Transport, Thermodynamics in Biomedical Research
Fall 1998	BMB 596 Molecular Biology/Biochemistry Student Seminar (with G. Woldegiorgis)
Spring 1999	BMB 529 Biochemistry III: Metabolism and Bioenergetics (with G. Woldegiorgis and V. Renganathan)
Winter 2000	BMB 596 Molecular Biology/Biochemistry Student Seminar (with G. Woldegiorgis)
Spring 2000	BMB 529 Biochemistry III: Metabolism and Bioenergetics (with G. Woldegiorgis and M. Ralle)
Winter 2001	BMB 532 Bioenergetics and Membrane Transport (with P. Paucek)
Spring 2001	BMB 529 Biochemistry III: Metabolism and Bioenergetics (with G. Woldegiorgis and M. Ralle)
Winter 2002	BMB 596 Molecular Biology/Biochemistry Student Seminar
Spring 2002	BMB 529 Biochemistry III: Metabolism and Bioenergetics (with G. Woldegiorgis)

Students

Ph.D.

   Nakashima, Richard A., 1982
   Martin, William H., 1984
   Nath, Sati, 1990
   Li, Xianqiang, 1990
   Sun, Xiaocheng, 1991
   Orosz, David E., 1993
   Shariat-Madar, Zia, 1993
   Weihua Li, 1993
   Hongfa Zhu, 1996
   Martin Modriansky, 1997
   Vladimir Yarov-Yarovoy, 1998
   Martin Jaburek, 1999

M.S.

Qiya Zhang, 1993
Cheng, Hailiang, 1995
Stueve, Thomas K., 1995
Nan Xu, 1996
Baoyu Lin, 1997
Key Tse, 2002

Fellows

Peter Schindler, Visiting Student, 1994-1995, supported by a grant from E. Merck, Darmstadt, Germany.

Robert S. Dordick, Ph.D., Miami, 1979-1981, Research Scientist, Dow Chemical Corp.

Andrew D. Beavis, Ph.D., 1982-1984, Associate Professor, Department of Pharmacology, Medical College of Ohio.

    Daniel J. DiResta, Ph.D., 1983-1987, Assistant Professor, Department of Biology, University of Miami.

Petr Jezek, Ph.D., 1988-1991, Visiting Scientist 1993-1995, Scientist, Institute of Physiology, Czechoslovak Academy of Sciences, Prague.

Petr Paucek, Ph.D., 1991-1996. Research Scientist, Oregon Graduate Institute.

   Dennis Schultz, Ph.D., 1993-1994, Oregon Health Sciences University.

   Valeri Zinchenko, Ph.D., 1993-1994, Institute of Cell Biophysics, Academy of Sciences, Pushchino, Moscow Region, Russia.

   Shize Lei, Ph.D., 1993-1994, Department of Pediatrics, Wenjiang County People's Hospital, Chengdu, Sichuan, P.R. China.

   Stefano Vassanelli, Ph.D., 1993-1994, Università degli Studi di Padova, Dipartimento di Scienze Biomediche Sperimentali, Padova,         Italy.

Xiaowei Zhang, Ph.D., 1993-1995, Oregon Health Sciences University, Portland, OR.

Robert Bajgar, B.S., Visiting Student, 1998-2000, Palacky University, Olomouc, Czech Republic.

Miroslav Varecha, B.S., Visiting Student, 1998-2000, Palacky University, Olomouc, Czech Republic.

Alicia Kowaltowski, Ph.D., 1999-2000.

Subramaniam Seetharaman, M.D., 1998-2002.

Professionally-related Service

Editorial Boards

    Journal of Bioenergetics and Biomembranes
    Molecular and Cellular Biochemistry
    Journal of Biological Chemistry, 1994-1999
    Journal of Molecular and Cellular Cardiology, 2000-present

National and Regional Committees

    Physical Biochemistry Study Section, NIH, ad hoc
    Review panel, Cellular Biochemistry Program, NSF, 1989-1991
    Chair, Service Area Research Advisory Committee AHA, 1989-1990
    Research Steering Committee, Ohio AHA, 1990-1992
    Cardiovascular Merit Review Board, VA, 1991-1993
    Executive Board Member, Bioenergetics Subgroup of Biophysical Society, 1990- 1993

Memberships in Professional Societies

    American Association for the Advancement of Science
    American Society of Biological Chemistry and Molecular Biology
    American Society for Pharmacology and Experimental Therapeutics
    Biophysical Society
    Biophysical Society Subgroups: Bioenergetics and Membrane Biophysics
    International Society for Heart Research
    American Heart Association